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Nannochloropsis, a rich source of diacylglycerol acyltransferases for engineering of triacylglycerol content in different hosts.

Biotechnol Biofuels. 2017; 
Zienkiewicz Krzysztof,Zienkiewicz Agnieszka,Poliner Eric,Du Zhi-Yan,Vollheyde Katharina,Herrfurth Cornelia,Marmon Sofia,Farré Eva M,Feussner Ivo,Benning Chris
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Recombinant Proteins ]. Protein separation and immunoblot analysis with primary His-tag antibody (GenScript, Piscataway, NJ Get A Quote

摘要

Photosynthetic microalgae are considered a viable and sustainable resource for biofuel feedstocks, because they can produce higher biomass per land area than plants and can be grown on non-arable land. Among many microalgae considered for biofuel production, (CCMP1779) is particularly promising, because following nutrient deprivation it produces very high amounts of triacylglycerols (TAG). The committed step in TAG synthesis is catalyzed by acyl-CoA:diacylglycerol acyltransferase (DGAT). Remarkably, a total of 13 putative DGAT-encoding genes have been previously identified in CCMP1779 but most have not yet been studied in detail.

关键词

DGAT,Lipid droplets,Lipid storage,Microalgae,Nannochloropsis oceanica,Triacylglyc