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Proteins, Expression, Isolation and Analysis | .... lysates on glutathione-agarose or -sepharose (Sigma; GenScript) and eluted with 20 mM glutathione.... with purified, recombinant GST-fusion proteins immobilized on glutathione sephar- ose beads (GenScript | Get A Quote |
Local actin filament formation powers the development of the signal-receiving arbor of neurons that underlies neuronal network formation. Yet, little is known about the molecules that drive these processes and may functionally connect them to the transient calcium pulses observed in restricted areas in the forming dendritic arbor. Here we demonstrate that Cordon-Bleu (Cobl)-like, an uncharacterized protein suggested to represent a very distantly related, evolutionary ancestor of the actin nucleator Cobl, despite having only a single G-actin-binding Wiskott-Aldrich syndrome protein Homology 2 (WH2) domain, massively promoted the formation of F-actin-rich membrane ruffles of COS-7 cells and of dendritic... More