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Ethylene production with engineered Synechocystis sp PCC 6803 strains.

Microb. Cell Fact.. 2017; 
Veetil Vinod Puthan,Angermayr S Andreas,Hellingwerf Kla
Products/Services Used Details Operation
Codon Optimization ....] and the codon usage table described in [32]. The gene was synthesized by GenScript (NJ, USA) and delivered.... and directly inserted into pHKH001 by GenScript. The efe gene amplified with the Pbs-30-containing primer Get A Quote

摘要

Metabolic engineering and synthetic biology of cyanobacteria offer a promising sustainable alternative approach for fossil-based ethylene production, by using sunlight via oxygenic photosynthesis, to convert carbon dioxide directly into ethylene. Towards this, both well-studied cyanobacteria, i.e., Synechocystis sp PCC 6803 and Synechococcus elongatus PCC 7942, have been engineered to produce ethylene by introducing the ethylene-forming enzyme (Efe) from Pseudomonas syringae pv. phaseolicola PK2 (the Kudzu strain), which catalyzes the conversion of the ubiquitous tricarboxylic acid cycle intermediate 2-oxoglutarate into ethylene.

关键词

Arginine,Cyanobacteria,Ethylene,Glycogen,Oxoglutarate,Sustainable,Synechocy