Collective cell migration of without cAMP oscillations at multicellular stages.

In ， a model organism for the study of collective cell migration， extracellular cyclic adenosine 3'，5'-monophosphate (cAMP) acts as a diffusible chemical guidance cue for cell aggregation， which has been thought to be important in multicellular morphogenesis. Here we revealed that the dynamics of cAMP-mediated signaling showed a transition from propagating waves to steady state during cell development. Live-cell imaging of cytosolic cAMP levels revealed that their oscillation and propagation in cell populations were obvious for cell aggregation and mound formation stages， but they gradually disappeared when multicellular slugs started to migrate. A similar transition of signaling dynamics occurred with phosphatidylinositol 3，4，5-trisphosphate signaling， which is upstream of the cAMP signal pathway. This transition was programmed with concomitant developmental progression. We propose a new model in which cAMP oscillation and propagation between cells， which are important at the unicellular stage， are unessential for collective cell migration at the multicellular stage.