Products/Services Used | Details | Operation |
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Recombinant Proteins | … We coated NUNC MaxiSorp 96-well plates (Thermo Scientific) with 1 μg/mL murine Fshr extracellular domain (GenScript) in PBS overnight at 4°C. We washed the plates with PBS-0.5% Tween20 and blocked with PBS-10% fetal bovine serum (FBS) for 1 hr at room temperature … | Get A Quote |
Ovarian cancer presents in 80% of patients as a metastatic disease, which confers it with dismal prognosis despite surgery and chemotherapy. However, it is an immunogenic disease, and the presence of intratumoral T?cells is a major prognostic factor for survival. We used a synthetic consensus (SynCon) approach to generate a novel DNA vaccine that breaks immune tolerance to follicle-stimulating hormone receptor (FSHR), present in 50% of ovarian cancers but confined to the ovary in healthy tissues. SynCon FSHR DNA vaccine generated robust CD8 and CD4 cellular immune responses and FSHR-redirected antibodies. The SynCon FSHR DNA vaccine delayed the progression of a highly aggressive ovarian cancer model wit... More