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Splitomicin， a SIRT1 Inhibitor， Enhances Hematopoietic Differentiation of Mouse Embryonic Stem Cells.

Int J Stem Cells. 2019-03;

ParkJeong-A，ParkSangkyu，ParkWoo-Youn，HanMyung-Kwan，LeeYoun

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Peptide Synthesis	… On day 7, to ensure the viability of the primary differentiation cultures over an extended period of time, feed medium (IMDM, 0.5% methylcellulose, 15% FBS, 150 μM MTG, 160 ng/ml mouse SCF, 30 ng/ml mouse IL-3 (PeproTech), 20 ng/ml human IL-6 (Genscript, Scotch Plains …	Get A Quote

摘要

Embryonic stem (ES) cells have pluripotent ability to differentiate into multiple tissue lineages. SIRT1 is a class III histone deacetylase which modulates chromatin remodeling， gene silencing， cell survival， metabolism， and development. In this study， we examined the effects of SIRT1 inhibitors on the hematopoietic differentiation of mouse ES cells.