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Longitudinal Analysis of the Human B Cell Response to Ebola Virus Infection.

Cell. 2019-05;

DavisCarl W，JacksonKatherine J L，McElroyAnita K，HalfmannPeter，HuangJessica，ChennareddyChakravarthy，PiperAshley E，LeungYvonne，Albari?oCésar G，CrozierIan，EllebedyAli H，SidneyJohn，SetteAlessandro，YuTianwei，NielsenSandra C A，GoffArthur J，SpiropoulouChristina F，SaphireErica Ollman，CavetGuy，KawaokaYoshihiro，MehtaAneesh K，GlassPamela J，BoydScott D，Ahmed

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摘要

Ebola virus (EBOV) remains a public health threat. We performed a longitudinal study of B cell responses to EBOV in four survivors of the 2014 West African outbreak. Infection induced lasting EBOV-specific immunoglobulin G (IgG) antibodies， but their subclass composition changed over time， with IgG1 persisting， IgG3 rapidly declining， and IgG4 appearing late. Striking changes occurred in the immunoglobulin repertoire， with massive recruitment of naive B cells that subsequently underwent hypermutation. We characterized a large panel of EBOV glycoprotein-specific monoclonal antibodies (mAbs). Only a small subset of mAbs that bound glycoprotein by ELISA recognized cell-surface glycoprotein. However， th... More