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Proteins, Expression, Isolation and Analysis | … Chimeric con- structs of CCR5 containing the extracellular domains of CCR1 and “reverse” receptor constructs of CCR1 containing extracellular do- mains of CCR5 (▶Figure 1 B) were designed in-house and pur- chased from GenScript USA Inc … | Get A Quote |
The chemokine CCL5 recruits monocytes into inflamed tissues by triggering primarily CCR1-mediated arrest on endothelial cells, whereas subsequent spreading is dominated by CCR5. The CCL5-induced arrest can be enhanced by heteromer formation with CXCL4. To identify mechanisms for receptor-specific functions, we employed CCL5 mutants and transfectants expressing receptor chimeras carrying transposed extracellular regions. Mutation of the basic 50s cluster of CCL5, a coordinative site for CCL5 surface presentation, reduced CCR5- but not CCR1-mediated arrest and transmigration. Impaired arrest was restored by exchanging the CCR5-N-terminus for that of CCR1, which supported arrest even without the 50s clus... More