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Proteins, Expression, Isolation and Analysis | … Equal amounts (25 μg) of proteins were used for separation by electrophoresis in 4–20% ExpressPLUS TM PAGE gels (GenScript, USA) and then transferred to PVDF membranes for 70 minutes. The membranes were incubated with the primary antibody (1:1,000) overnight … | Get A Quote |
C-X-C motif chemokine ligand 2 (CXCL2) is a small secreted protein that exhibits a structure similar to the proangiogenic subgroup of the CXC chemokine family. Recently, accumulating evidence suggests that chemokines play a pivotal role in cancer progression and carcinogenesis. We examined the expression levels of 7 types of ELR+ CXCLs messenger RNA (mRNA) in 264 clinical samples. We found that CXCL2 expression was stably down-regulated in 94% of hepatocellular carcinoma (HCC) specimens compared with paired adjacent normal liver tissues and some HCC cell lines. Moreover, CXCL2 overexpression profoundly attenuated HCC cell proliferation and growth and induced apoptosis in vitro. In animal studies, we fou... More