Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | … thiogalactoside (Sigma). The fused proteins were obtained from inclusion body and purified by avidity chromatography on a Ni 2+ -agarose column (GenScript, USA). The particles were obtained after routine renaturation. Endotoxin … | Get A Quote |
The present study aims at establishing a novel vaccine procedure based on HBc-VLP-pulsed DCs. Immature mice BMDCs could capture HBc-VLP or HBc-VLP packaging CpG efficiently and present the antigen to syngeneic mice spleen T cells in vitro. Immunization with DCs showed that compared to VLP-pulsed DCs, VLP packaging CpG-pulsed DCs elicit stronger T-cell responses in vivo, as measured by both intracellular production of IFN-gamma and in vivo killing assays by Ag-specific T cells. In the B16-pIR-HH tumor therapy model, the growth of established tumors was significantly inhibited by single immunization of DCs pulsed with HBc-VLP packaged with CpG, resulting in significantly longer survival of immunized anima... More