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Proteins, Expression, Isolation and Analysis | … The purity of the purified LTA was determined by its endotoxin concentration and its protein level with the ToxinSensor Chromogenic Lumulus Amebocyte Lysate Endotoxin Assay kit (Genscript, USA) and by silver staining after SDS-PAGE gel loading … | Get A Quote |
Human skin is continuously exposed to ultraviolet (UV)-induced photoaging. UVA increases the activity of MMP-1 in dermal fibroblasts through mitogen-activated protein kinase (MAPK), p38, signaling. The irradiation of keratinocytes by UVA results in the secretion of the inflammatory cytokine, tumor necrosis factor-α (TNF-α), and the stimulation of MMP-1 in normal human dermal fibroblasts (NHDFs). Lipoteichoic acid (LTA) is a component of the cell wall of gram-positive Lactobacillus spp. of bacteria. LTA is well known as an anti-inflammation molecule. LTA of the bacterium Lactobacillus plantarum has an anti-photoaging effect, but the potential anti-photoaging effect of the other bacteria has not been ... More