| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | GST fusion proteins were bound to glutathione sepharose beads (Genscript, Nanjing, China) and incubated with in vitro translated protein at 4°C for 2 h with gentle agitation in binding buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 10% glycerol, 1 mM EDTA, 1 mM DTT, 0.2% NP-40). | Get A Quote |
Many diseases are transmitted by mosquitoes， including malaria， dengue fever， yellow fever， filariasis， and West Nile fever. Chemical control plays a major role in managing mosquito-borne diseases. However， excessive and continuous application of insecticides has caused the development of insecticide resistance in many species including mosquito， and this has become the major obstacle to controlling mosquito-borne diseases. Insecticide resistance is the result of complex polygenic inheritance， and the mechanisms are not well understood. Ribosomal protein RPS29 was found to be associated with DM resistance in our previous study. In this study， we aim to further investigate the involvement of RP... More
