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Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts.

Biotechnol Biofuels. 2018;

WangLaiyou，DengAihua，ZhangYun，LiuShuwen，LiangYong，BaiHua，CuiDi，QiuQidi，ShangXiuling，YangZhao，HeXiuping，WenTi

Products/Services Used	Details	Operation
Codon Optimization	4CL gene encoding 4-coumaryl-CoA ligase from Arabidopsis thaliana, STS gene encoding stilbene synthase from Vitis vinifera, and human serum albumin gene HSA were all synthesized by GenScript (Nanjing, China) in codon-optimized versions for expression in O. polymorpha.	Get A Quote

摘要

The thermotolerant methylotrophic yeast has been regarded as an important organism for basic research and biotechnological applications. It is generally recognized as an efficient and safe cell factory in fermentative productions of chemicals， biofuels and other bio-products. However， it is difficult to genetically engineer for the deficiency of an efficient and versatile genome editing technology.

关键词

CRISPR–Cas9-assisted multiplex genome editing，Markerless multi-copy integration，Markerless multi-locus integration，Ogataea polymorpha，Saccharomyces cerevi

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