Maturation of the cytochrome cd1 nitrite reductase NirS from Pseudomonas aeruginosa requires transient interactions between the three proteins NirS， NirN and NirF.

The periplasmic cytochrome cd1 nitrite reductase NirS occurring in denitrifying bacteria such as the human pathogen Pseudomonas aeruginosa contains the essential tetrapyrrole cofactors haem c and haem d1. Whereas the haem c is incorporated into NirS by the cytochrome c maturation system I， nothing is known about the insertion of the haem d1 into NirS. Here， we show by co-immunoprecipitation that NirS interacts with the potential haem d1 insertion protein NirN in vivo. This NirS-NirN interaction is dependent on the presence of the putative haem d1 biosynthesis enzyme NirF. Further， we show by affinity co-purification that NirS also directly interacts with NirF. Additionally， NirF is shown to be a membrane anchored lipoprotein in P. aeruginosa. Finally， the analysis by UV-visible absorption spectroscopy of the periplasmic protein fractions prepared from the P. aeruginosa WT (wild-type) and a P. aeruginosa ΔnirN mutant shows that the cofactor content of NirS is altered in the absence of NirN. Based on our results， we propose a potential model for the maturation of NirS in which the three proteins NirS， NirN and NirF form a transient， membrane-associated complex in order to achieve the last step of haem d1 biosynthesis and insertion of the cofactor into NirS.