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Proteins, Expression, Isolation and Analysis | Finally, exosome pellets were resuspend in 1/10 original volume of nuclease-free water, 25 μL 9% sucrose containing protease inhibitors (GenScript Biotechnology, Nanjing, China) was added, and the preparation was stored at −4°C. | Get A Quote |
Exosomes, membranous nanovesicles, naturally carry bio-macromolecules or miRNA and play impoetant roles in tumor pathogenesis. Here, we showed that macrophages cell-derived exosomes can function as vehicles to deliver exogenous miR-21 inhibitor into BGC-823 gastric cancer cells. Exosomes loaded with miR-21 inhibitor significantly increased miR-21 levels in BGC-823, but miR-21 inhibitor loaded in exosomes exerted an opposite effect. miRNA transfected with exosomes had less cellular toxicity to host cells compared to conventional transfection methods. The miR-21 inhibitor loaded exosomes promoted the migration ability and reduced apoptosis of BGC-823 gastric cancer cells. These observations indicate that ... More