Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in .

The bacterial CRISPR/Cas genome editing system has provided a major breakthrough in molecular biology. One use of this technology is within a nuclease-based gene drive. This type of system can install a genetic element within a population at unnatural rates. Combatting of vector-borne diseases carried by metazoans could benefit from a delivery system that bypasses traditional Mendelian laws of segregation. Recently， laboratory studies in fungi， insects， and even mice， have demonstrated successful propagation of CRISPR gene drives and the potential utility of this type of mechanism. However， current gene drives still face challenges including evolved resistance， containment， and the consequences of application in wild populations. Additional research into molecular mechanisms that would allow for control， titration， and inhibition of drive systems is needed.