Global proteogenomic analysis of human MHC class I-associated peptides derived from non-canonical reading frames.

In view of recent reports documenting pervasive translation outside of canonical protein-coding sequences， we wished to determine the proportion of major histocompatibility complex (MHC) class I-associated peptides (MAPs) derived from non-canonical reading frames. Here we perform proteogenomic analyses of MAPs eluted from human B cells using high-throughput mass spectrometry to probe the six-frame translation of the B-cell transcriptome. We report that ∼ 10% of MAPs originate from allegedly noncoding genomic sequences or exonic out-of-frame translation. The biogenesis and properties of these 'cryptic MAPs' differ from those of conventional MAPs. Cryptic MAPs come from very short proteins with atypical C termini， and are coded by transcripts bearing long 3'UTRs enriched in destabilizing elements. Relative to conventional MAPs， cryptic MAPs display different MHC class I-binding preferences and harbour more genomic polymorphisms， some of which are immunogenic. Cryptic MAPs increase the complexity of the MAP repertoire and enhance the scope of CD8 T-cell immunosurveillance.