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Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein.

PLoS ONE. 2009;

VolkmannGerrit，LiuXiang

Products/Services Used	Details	Operation
Proteins, Expression, Isolation and Analysis	After washing away unbound proteins with oSB, the resin was incubated in oSB containing 0.1 mM TCEP and either 45 µM IC-L (for MINC protein) or 125 µM IC-B (for EINC protein, sequence: GVFVHNSAGSK-biotin, purchased from GenScript), and the incubation was continued overnight at room temperature.	Get A Quote

摘要

Site-specific protein labeling or modification can facilitate the characterization of proteins with respect to their structure， folding， and interaction with other proteins. However， current methods of site-specific protein labeling are few and with limitations， therefore new methods are needed to satisfy the increasing need and sophistications of protein labeling.