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De novo synthesis of trans-10， cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia lipolytica.

Microb. Cell Fact.. 2012;

ZhangBaixi，RongChunchi，ChenHaiqin，SongYuanda，ZhangHao，Che

Products/Services Used	Details	Operation
Codon Optimization	The codon-optimized sequence (oPAI) was designed according to the codon preference of Y. lipolytica using the GenScript OptimumGeneTM system. GCCACA was added before the first methionine to create a Kozak sequence. The newly designed DNA sequence flanked with PmlI and KpnI was synthesized (GenScript, Nanjing, China).	Get A Quote

摘要

Conjugated linoleic acid (CLA) has many well-documented beneficial physiological effects. Due to the insufficient natural supply of CLA and low specificity of chemically produced CLA， an effective and isomer-specific production process is required for medicinal and nutritional purposes.