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Ribosome display for the rapid generation of high-affinity Zika-neutralizing single-chain antibodies.

PLoS ONE. 2018;

KunamneniAdinarayana，YeChunyan，BradfuteSteven B，Durvasula

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摘要

Zika virus (ZIKV) is an emerging pathogen with no approved therapeutics and only limited diagnostics available. To address this gap， six mouse single-chain antibodies (scFvs) to ZIKV envelope (E) protein were isolated rapidly and efficiently from a ribosome-displayed antibody library constructed from the spleens of five immunized mice.