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Proteins, Expression, Isolation and Analysis | cells were treated by ChemDiv-3 (20 μM) and cultured for 24 h. The formation of sE-dimer was measured with Western Blotting in the absence of reducing agent DTT by rabbit anti-strep-tag II polyclone antibody (GenScript | Get A Quote |
Flaviviruses are emerging arthropod-borne viruses posing a great threat to human beings worldwide. The E dimer configuration of the flavivirus was prominent during viral assembly, maturation and entry. Neutralization antibodies targeting E dimer played the important role in controlling the flavivirus infection. Previously, the ideal drug target of small molecular inhibitors of JEV was viral proteases and polymerases. The crystal structure of JEV E protein showed a conserved pocket in it is important at membrane fusion step. Recently, a set of anti-virus drugs has been found by virtual screening. Here, we show that the fusion-loop pocket of JEV E protein was a conservative region and an ideal drug target... More