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Proteins, Expression, Isolation and Analysis | … Two commercially prepared plasmids with known HIVDR mutations, P1 (G73T_K103N) and P2 (G73T_K65R) (Genscript, Piscataway, USA), were used to assess the error rate of the assay, as well as the limit of detection for minority variants in a range of P1:P2 mixtures … | Get A Quote |
Conventional HIV drug resistance (HIVDR) genotyping utilizes Sanger sequencing (SS) methods, which are limited by low data throughput and the inability of detecting low abundant drug resistant variants (LADRVs). Here we present a next generation sequencing (NGS)-based HIVDR typing platform that leverages the advantages of Illumina MiSeq and HyDRA Web. The platform consists of a fully validated sample processing protocol and HyDRA web, an open web portal that allows automated customizable NGS-based HIVDR data processing. This platform was characterized and validated using a panel of HIV-spiked plasma representing all major HIV-1 subtypes, pedigreed plasmids, HIVDR proficiency specimens and clinical speci... More