| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | … Then the activity of isolated serums was examined by ELISA assay using a commercially available M2e, human (SLLTEVETPIRNEWGCRCNDSSD) peptide as coating antigen (Genscript, USA) and anti-IgY Peroxidase (Genscript, USA) as the secondary antibody. 3. Results … | Get A Quote |
Small peptides require large carriers to stimulate the humoral immune system. The filamentous phages， such as M13， have been proposed as vectors for expressing and carrying these peptides on their capsid surface. M2e 2-9 (SLLTEVET) residues of the transmembrane protein M2 of Influenza A virus are conserved and considered as a suitable target for immunization against a wide range of Influenza A virus strains. Here， M2e (2-9) sequence of Influenza A virus was fused to the N-terminus of the major coat protein gpVIII of M13 phage and was used to immunize broiler chickens. The results showed that the SLLTEVET peptide on the surface of M13 phage was expressed， the hybrid phage was immunogenic and produced spe... More
