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Proteins, Expression, Isolation and Analysis | … Hamster IgG (5µg/ml) overnight at 4ºC. Lysates were then incubated with protein G sepharose beeds (GenScript, Piscataway, NJ, USA) for 2 h at room temperature. Beeds were collected, washed three times with RIPA buffer without protease inhibitor, and boiled in SDS Page 8 … | Get A Quote |
Aberrant expression of the glycoprotein mucin-1 (MUC1) has been associated with pancreatic cancer progression and metastasis as a result of mediating the oncogenic transcriptional regulation of target genes. In the present study, we demonstrate that MUC1 downregulates the expression of the tumor suppressor polypeptide N-acetylgalactosaminyltransferase 5 in pancreatic cancer. ChIP-on-chip analysis revealed that the MUC1 cytoplasmic tail binds to regulatory elements in the GALNT5 gene. Additionally, MUC1 increases binding of p53 and c-Jun and decreases the binding of Sp1 to the proximal promoter and exonic regions of GALNT5. We also observed that expression of N-acetylgalactosaminyltransferase 5 is inversiona... More