| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | Total proteins from the transformation leaves were incubated with GST-Resin (GenScript, USA). After washing, the bound proteins were eluted and detected by western blotting using anti-GFP (Genscript, USA), and anti-GST antibodies (Genscript, USA)...For GST pull-down assay, the GST-fusion proteins (GST-JAZs and GST-MAPKs) were co-incubated with MBP-IbbHLH3 and GST-Resin (GenScript, USA). After washing with PBS buffer, the GST-binding protein was eluted and detected by western blotting using anti-GST and anti-MBP antibodies (GenScript, USA). | Get A Quote |
IbNAC1 is known to activate the defense system by reprogramming a genetic network against herbivory in sweet potato. This regulatory activity elevates plant defense potential but relatively weakens plants by IbNAC1-mediated JA response. The mechanism controlling IbNAC1 expression to balance plant vitality and survival remains unclear. In this study， a wound-responsive G-box cis-element in the IbNAC1 promoter from -1484 to -1479 bp was identified. From a screen of wound-activated transcriptomic data， one transcriptional activator， IbbHLH3， and one repressor， IbbHLH4， were selected that bind to and activate or repress， respectively， the G-box motif in the IbNAC1 promoter to modulate the IbNAC1-med... More
