Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | … used. Unmodified as well as N-terminally biotinylated (Biotin-Ahx) human peptides were purchased from Genscript: UFD1 ( 221 GELGFRAFSGSGNRLDGKKKG 241 ), gp78 ( 622 VTLRRRMLAAAAERRLQKQ 640 ). Protein | Get A Quote |
The type II AAA ATPase p97 interacts with a large number of cofactors that regulate its function by recruiting it to different cellular pathways. Most of the cofactors interact with the N-terminal (N) domain of p97, either via ubiquitin-like domains or short linear binding motifs. While some linear binding motifs form α helices, another group features short stretches of unstructured hydrophobic sequences as found in the so-called SHP (BS1, binding segment 1) motif. Here we present the crystal structure of a SHP-binding motif in complex with p97, which reveals a so far uncharacterized binding site on the p97 N domain that is different from the conserved binding surface of all other known p97 cofactors.... More