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Adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose.

Microb. Cell Fact.. 2016;

LeeJungseok，SaddlerJack N，UmYoungsoon，WooHan

Products/Services Used	Details	Operation
Codon Optimization	Each target gene was synthesized (Genscript, USA) with codon-optimization (Gene Designer 2.0 software [28]) for C. glutamicum and was assembled using a standard BglBrick cloning method	Get A Quote

摘要

An efficient microbial cell factory requires a microorganism that can utilize a broad range of substrates to economically produce value-added chemicals and fuels. The industrially important bacterium Corynebacterium glutamicum has been studied to broaden substrate utilizations for lignocellulose-derived sugars. However， C. glutamicum ATCC 13032 is incapable of PTS-dependent utilization of cellobiose because it has missing genes annotated to β-glucosidases (bG) and cellobiose-specific PTS permease.