Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | … metalloproteinase (MMP)—sensitive peptide. A peptide sequence ( GCREGPQGIWGQERCG; 1733 Da) (GenScript USA Inc., Piscataway, NJ) degradable by a range of MMP's (24) was used to form 3.5% (mass/mass) gels. In order to … | Get A Quote |
Directed cell motility, as controlled by soluble factors, is crucial for many biological processes, including development, cancer progression, and wound healing. The use of directed cell motility also shows promise for applications in regenerative medicine such as therapeutic angiogenesis. Unfortunately, current in vitro 3-D migration and invasion models limit our understanding and application of these processes. Here, we present a novel and cost-effective 3-D chemotaxis assay for assessing the invasive response of cells to a chemoattractant extracellular matrix (ECM). Our system takes advantage of a custom-casting chamber to set two gels in contact with each other along a defined front, one con... More