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Optimization of Fermentation Conditions for Reteplase Expression by Using Response Surface Methodology.

Avicenna J Med Biotechnol. 2016-03;

ZareHamze，Mir Mohammad SadeghiHamid，AkbariVa

Products/Services Used	Details	Operation
Codon Optimization	… efficiency and mRNA stability. Software is available to design an optimized sequence for expression in a particular host (OPTIMIZER, Puigbo et al. 2007 or OptimumGene from Genscript). Several companies offer the possibility …	Get A Quote

摘要

Expression of heterologous proteins at large scale is often a challenging job due to plasmid instability， accumulation of acetate and oxidative damage in bioreactors. Therefore， it is necessary to optimize parameters influencing cell growth and expression of recombinant protein.