| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | The nucleotide sequence for SferH-1 mature protein was codon-optimized according to the codon-usage frequencies of Arabidopsis, and synthesized with a BglII restriction cut site at the 5′-end and a BstEII cut site at the 3′-end by GenScript (Piscataway, NJ). | Get A Quote |
Plant lignocellulosic biomass is an abundant， renewable feedstock for the production of biobased fuels and chemicals. Previously， we showed that iron can act as a co-catalyst to improve the deconstruction of lignocellulosic biomass. However， directly adding iron catalysts into biomass prior to pretreatment is diffusion limited， and increases the cost of biorefinery operations. Recently， we developed a new strategy for expressing iron-storage protein ferritin intracellularly to accumulate iron as a catalyst for the downstream deconstruction of lignocellulosic biomass. In this study， we extend this approach by fusing the heterologous ferritin gene with a signal peptide for secretion into cell walls (... More
