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Proteins, Expression, Isolation and Analysis | … For flagellin treatment, cotyledons of 14-d-old plants grown in Perlite were infiltrated with 1 µM flagellin 22 (flg 22; QRLSTGSRINSAKDDAAGLQIA; GenScript, Piscataway, USA) solution using a 1 cm3 plastic syringe Control plants were treated with inactive peptide ∆flg22 … | Get A Quote |
Phospholipase D (PLD) and its product phosphatidic acid are now considered to be one of the key elements of numerous physiological processes in plants including the salicylic acid signalling pathway. The presented study investigates the transcriptional regulation of Brassica napus PLDs following treatments with defense-related stimuli. We cloned eight B. napus genes encoding members of PLDβ, γ, and δ isoforms and performed phylogenetic analysis with its ancestor species Brassica rapa and Brassica oleracea, and with the model plant Arabidopsis thaliana. Transcription of the identified genes was monitored after treatment with benzothiadiazole (BTH), methyl jasmonate (MeJA), bacterial elicitor flg22, wounding, ... More