| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … Both peptides were obtained from GenScript (Piscataway, NJ) All hydrogels were synthesized by reaction at room temperature for 30 min to yield a final concentration of 2 wt% MaHA and 2 mM RGD, with a theoretical equimolar ratio between cysteines and maleimide groups … | Get A Quote |
In order to achieve bone repair， bone morphogenetic protein-2 (BMP-2) is typically delivered in non-physiological doses and can result in significant adverse side effects. To reduce the amount of BMP-2 necessary for bone formation， we delivered a known chemokine (stromal cell derived factor-1α， SDF-1α) in combination with BMP-2 using proteolytically degradable hydrogels. A critical-sized calvarial defect was used to determine the effect of biomolecule delivery on bone formation in vivo. The treatment group with combined SDF-1α and BMP-2 hydrogel delivery showed significantly higher bone formation when compared to hydrogels loaded with the same BMP-2 or SDF-1α concentrations alone， suggesting the com... More
