Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | … Materials and Methods Lactoferrins and BCG Recombinant mouse LF (mLF) was produced in CHO stable cell lines and provided by PharmaReview Corporation (Houston, TX), manufactured under endotoxin free, serum-free conditions (Genscript USA) [42,43] … | Get A Quote |
Lactoferrin has been investigated for its adjuvant action to boost the BCG vaccine. Previous studies demonstrated that lactoferrin (LF) enhanced efficacy of the Bacillus Calmette-Guérin (BCG) vaccine to protect mice against the virulent Erdman Mycobacterium tuberculosis challenge. The studies here investigate the hypothesis that a novel CHO-derived recombinant mouse LF can modify cytokine production and antigen presentation molecules on macrophages. The mouse LF (rmLF) was examined for effects on bone marrow derived macrophage (BMM) activities when cultured with BCG. Comparisons were made to CHO-derived recombinant human LF (rhLF). Inflammatory cytokine responses were investigated, as were antigen presentati... More