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Proteins, Expression, Isolation and Analysis | … GAPDH and Lamin A/C (Genscript, Piscataway, NJ) were used as loading controls … Nuclear and cytoplasmic extracts were analyzed by Western blot GAPDH (Santa Cruz) and Lamin A/C (Genscript, Piscataway, NJ) were used as loading and cell compartment controls … | Get A Quote |
The synthetic 15-mer arginine-glycine-aspartic acid (RGD) domain of osteopontin (OPN) is protective in vitro and in vivo against dopaminergic cell death and this protective effect may be mediated through interaction with integrin receptors to regulate neurotrophic factor levels. We now examine this concept in rat primary ventral mesencephalic (VM) cultures. 1-Methyl-4-phenylpyridinium (MPP+) exposure reduced tyrosine hydroxylase (TH)-positive cell number and activated glial cells as shown by increased glial fibrillary acidic protein (GFAP), oxycocin-42 (OX-42) and ectodermal dysplasia 1 (ED-1) immunoreactivity. Both OPN and the RGD domain of OPN were equally protective against MPP+ toxicity in VM cultures and... More