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Proteins, Expression, Isolation and Analysis | … Mouse anti-tau antibody (Tau-5) and rabbit anti-Atg5 antibody were from Millipore (Billerica, MA, USA) Mouse anti-β tubulin was from GenScript (Piscataway, NJ, USA) Rabbit anti-LC3 (for Western blot) was from Novus (Littleton, CO, USA) … | Get A Quote |
Magnaporthe oryzae causes rice blast disease and has become a model organism of fungal infections. M. oryzae can oxygenate fatty acids by 7,8-linoleate diol synthase, 10R-dioxygenase-epoxy alcohol synthase, and by a putative manganese lipoxygenase (Mo-MnLOX). The latter two are transcribed during infection. The open reading frame of Mo-MnLOX was deduced from genome and cDNA analysis. Recombinant Mo-MnLOX was expressed in Pichia pastoris and purified to homogeneity. The enzyme contained protein-bound Mn and oxidized 18:2n-6 and 18:3n-3 to 9S-, 11-, and 13R-hydroperoxy metabolites by suprafacial hydrogen abstraction and oxygenation. The 11-hydroperoxides were subject to β-fragmentation with formation ... More