| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | CNGC4-CaMBD-IQ (TQHFRYTFVNEKVKRSARYYSPG WRTWAAVAVQLAWRRYKHRLTLTS) peptides were purchased from GenScript and dissolved in water to make a 1 mM stock...Synthetic peptides (GenScript) and purified CaM were diluted in 25 mM Tris-Cl buffer (pH 7.5). CaM (200 pmol per reaction) was incubated with peptides of various molar ratios in 25 mM Tris-Cl buffer (pH 7.5) containing either 0.1 mM CaCl2 or 2 mM EGTA for 30 min at room temperature. | Get A Quote |
Pathogen-associated molecular patterns (PAMPs) activate innate immunity in both animals and plants. Although calcium has long been recognized as an essential signal for PAMP-triggered immunity in plants, the mechanism of PAMP-induced calcium signalling remains unknown1,2. Here we report that calcium nutrient status is critical for calcium-dependent PAMP-triggered immunity in plants. When calcium supply is sufficient, two genes that encode cyclic nucleotide-gated channel (CNGC) proteins, CNGC2 and CNGC4, are essential for PAMP-induced calcium signalling in Arabidopsis3-7. In a reconstitution system, we find that the CNGC2 and CNGC4 proteins together-but neither alone-assemble into a functional calcium channel th... More
