Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | … Proteins were separated by SurePAGE™ precast polyacrylamide gels with a gradient between 4% and 20% (GenScript, Nanjing, China) and transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). After … | Get A Quote |
Neoadjuvant chemoradiotherapy (nCRT) has been widely applied to improve the local control rate and survival rate in patients with locally advanced rectal cancer (LARC), yet only part of LARC patients would benefit from nCRT. Therefore, it is imperative to predict the therapeutic outcome of nCRT. Here, we showed that RAD18, an E3 ubiquitin-linked enzyme, played a fundamental role in predicting the response of LARC patients to nCRT. According to clinical data, patients with low RAD18 expression level in their pre-nCRT biopsies had a superior response to nCRT compared to those with high RAD18 expression. Inhibition of RAD18 expression in rectal cancer cells pronouncedly attenuated the proliferation and promoted ap... More