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Peptide Synthesis | … The disome is formed by using mutant eRF1 that is defective in peptide-releasing activity, and cryoelectron microscopy (cryo-EM) analysis of the disome revealed that the colliding ribosome is in a rotated form (Juszkiewicz et al., 2018) …DYKDDDDK peptide GenScript... | Get A Quote |
18S non-functional rRNA decay (NRD) eliminates non-functional 18S rRNA with deleterious mutations in the decoding center. Dissociation of the non-functional 80S ribosome into 40S and 60S subunits is a prerequisite step for degradation of the non-functional 18S rRNA. However, the mechanisms by which the non-functional ribosome is recognized and dissociated into subunits remain elusive. Here, we report that the sequential ubiquitination of non-functional ribosomes is crucial for subunit dissociation. 18S NRD requires Mag2-mediated monoubiquitination followed by Hel2- and Rsp5-mediated K63-linked polyubiquitination of uS3 at the 212 lysine residue. Determination of the aberrant 18S rRNA levels in sucrose grad... More