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Structure of a VH isolated from a naïve phage display library.

BMC Res Notes. 2019; 
WhiteBrandy,HuhIan,BrooksCo
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Codon Optimization … for V H H R419 [11] was codon optimized for E. coli expression and produced as a double stranded GenPart DNA fragment (Genscript Inc, NJ) … Fermentation was carried out overnight (16 h, 30 °C, 225 rev min −1 ). V H H R419 was extracted from the periplasm using an osmic … Get A Quote

摘要

To determine the X-ray structure and biophysical properties of a Camelid VH isolated from a naïve phage display library.

关键词

Nanobody,Single domain antibody