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Proteins, Expression, Isolation and Analysis | … Recombinant ADH was then purified by affinity column chromatography using His Bind Purification Kit (GE Healthcare, Pittsburgh, USA). Endotoxin was removed using ToxinErase TM Endotoxin Removal Kit (GenScript, Nanjing, China) and the concentration of endotoxin was … | Get A Quote |
proteins located on the cell wall and in the cytoplasm have gained great attention because they are not only involved in cellular metabolism and the maintenance of integrity but also interact with host immune systems. Previous research has reported that enolase from exhibits high immunogenicity and effectively protects mice against disseminated candidiasis. In this study, alcohol dehydrogenase (ADH) of was cloned and purified for the first time, and this study focused on evaluating its effects on the differentiation of the human monocytic cell line THP-1. The morphological features of THP-1 cells exposed to ADH were similar to those of phorbol-12-myristate acetate-differentiated (PMA-differentiated) macro... More