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Proteins, Expression, Isolation and Analysis | … Images were processed for brightness and contrast and assembled in Photoshop CS (Adobe, USA). 2.6 … rWSV181 was expressed in the supernatant and purified through His-Bind resin chromatography (GenScript, Nanjing, China) … | Get A Quote |
The Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway plays a critical role in host defense against viral infections. Here, we report the use of the Drosophila model system to investigate the modulation of the JAK/STAT pathway by the white spot syndrome virus (WSSV) protein WSV181. WSV181 overexpression in transgenic flies resulted in the downregulation of STAT92E and STAT92E-targeted genes. This result indicates that WSV181 can suppress JAK/STAT signaling by controlling STAT92E expression. An infection experiment was carried out on transgenic Drosophila infected with Drosophila C virus and on Litopenaeus vannamei injected with recombinant WSV181 and WSSV. The increased viral ... More