| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | … SIINFEKL or fluorescently labeled SIINFEK(FITC)L peptides (Genscript) were loaded at 100 µg mL −1 onto DC‐MVs by incubation at 37 °C … 5 × 10 5 cells were adoptively transferred into naïve congenic Thy1.2+ C57BL/6 mice (female, 6–8 weeks, Envigo, USA) via intravenous … | Get A Quote |
Cell membranes have recently gained attention as a promising drug delivery system. Here， dendritic cell membrane vesicles (DC-MVs) are examined as a platform to promote T cell responses. Nanosized DC-MVs are derived from DCs pretreated with monophosphoryl lipid A (MPLA)， a FDA-approved immunostimulatory adjuvant. These "mature" DC-MVs activate DCs in vitro and increase their expression of costimulatory markers. DC-MVs also promote cross-priming of antigen-specific T cells in vitro， increasing their survival and CD25 expression. In addition， these mature DC-MVs potently augment the expansion of adoptively transferred CD8+ T cells in vivo， generating twofold to fourfold higher frequency of antigen-speci... More
