Products/Services Used | Details | Operation |
---|---|---|
Proteins, Expression, Isolation and Analysis | … 2Program in Medical and Population Genetics and Metabolism Program, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA … SDS-PAGE and immunoblot analyses were performed, using the anti-ZnT10 (Abbexa) and anti-flag (GenScript) antibodies at dilutions of 1 … | Get A Quote |
Manganese (Mn) is extruded from the cell by the zinc transporter 10 (ZnT10). Loss of ZnT10 expression caused by autosomal mutations in the gene leads to hypermanganesemia in multiple organs. Here, combining fluorescent monitoring of cation influx in HEK293-T cells expressing human ZnT10 with molecular modeling of ZnT10 cation selectivity, we show that ZnT10 is exploiting the transmembrane Ca inward gradient for active cellular exchange of Mn In analyzing ZnT10 activity we used the ability of Fura-2 to spectrally distinguish between Mn and Ca fluxes. We found that () application of Mn-containing Ca-free solution to ZnT10-expressing cells triggers an influx of Mn, () reintroduction of Ca leads to cellular ... More