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Proteins, Expression, Isolation and Analysis | … The protein was dialyzed to remove imidazole, follow- ing by endotoxin removal using ToxinEraser™ Endotoxin Removal Kit (GenScript, China) and filter sterilization, then quantified using a BCA Protein Assay Reagent Kit (Pierce, USA) … | Get A Quote |
Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin's inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin's action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffiths... More