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Proteins, Expression, Isolation and Analysis | … These doses of endotoxin contamination, which below the detectable level of two sensitive Endotoxin Quantitation Kit (Genscript, China; Thermo, USA), were considered endotoxin free. We next examined the cellular localization of mhp390 in M. hyopneumoniae … | Get A Quote |
Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia (EP) and responsible for major economic losses in global swine industry. After colonization of the respiratory epithelium, M. hyopneumoniae elicits a general mucociliary clearance loss, prolonged inflammatory response, host immunosuppression and secondary infections. Until now, the pathogenesis of M. hyopneumoniae is not completely elucidated. This present study explores the pathogenicity of mhp390 (P68, a membrane-associated lipoprotein) by elucidating its multiple functions. Microtitrer plate adherence assay demonstrated that mhp390 is a new cilia adhesin that plays an important role in binding to swine tracheal cilia. Not... More