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Proteins, Expression, Isolation and Analysis | … All solvents and diluents used in this study were tested for endotoxin with the ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit following the manufacturer's protocol (GenScript, Piscataway, NJ,USA) as previously described by Adjei-Fremah et al … | Get A Quote |
In this study, the effect of polyphenolic extracts from cowpea (Vigna unguiculata) on global gene expression in bovine peripheral blood was investigated. Blood collected from Holstein-Friesian cows (n = 10) was treated with 10 µg/mL of cowpea phenolic extract (CPE) and subsequently used for transcriptional profiling using the Agilent bovine (v2) 4 × 44 K array. Calculation of fold change in gene expression and pathway analysis was conducted using the GeneSpring GX software 13.0. Real-time quantitative PCR was performed to validate the microarray data. Phenolic extracts of cowpea impacted global gene expression and resulted in 3170 differentially expressed genes (p < .05); 1716 genes were upregulated and 1454 ... More