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Distinct structural mechanisms determine substrate affinity and kinase activity of protein kinase Cα

ournal of Biological . 2018;

Sangbae Lee‡, Titu Devamani§, Hyun Deok Song‡, Manbir Sandhu‡ , Adrien Larsen‡ , Ruth Sommese§ , Abhinandan Jain¶ , Nagarajan Vaidehi‡, and Sivaraj Sivaramakrishnan§

Products/Services Used	Details	Operation
Peptide Synthesis	Reagents and peptides—Buffer chemicals were purchased from various commercial vendors and used without further purification. Peptides (1, 2, 4, 6, 9, 10, and 12) and the correspondingmutantpeptides(Figs.2and4)werecustom-synthesized by GenScript and solubilized in PKC buffer (20 mM HEPES(pH7.5),5mMMgCl2,0.5mMEGTA,and2mMDTT).	Get A Quote