Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | The ToxinSensor Chromogenic LAL Endotoxin Assay Kit was obtained from Genscript whereas the TLR7-agonist was purchased from Invivogen. | Get A Quote |
Here, we report on a strategy to encapsulate cancer cell lysate in immunomodulating microparticles in view of their potential use in personalized anticancer vaccination. In a first step, cancer cell lysate is entrapped into porous calcium carbonate (CaCO3) microparticles by a coprecipitation reaction. Second, a polymer substituted with a small molecule TLR7/8-agonist is adorbed onto the surface of the microparticles. Relative to soluble cell lysate, microparticle encapsulation yielded higher uptake of cell lysate by dendritic cells in vitro. Also, microparticle-adsorbed polymeric TLR7/8-agonists retained their TLRtriggering capacity resulting in immunogenic vaccine particles. We anticipate this strategy might h... More