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Nonredox thiolation in tRNA occurring via sulfur activation by a [4Fe-4S] cluster

Proceedings of the National Academy of Sciences. 2017;

Simon Arragain, Ornella Bimai, Pierre Legrand, Sylvain Caillat, Jean-Luc Ravanat, Nadia Touati, Laurent Binet, Mohamed Atta, Marc Fontecave, and Béatrice Golinelli-Pimpaneau

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Codon Optimization	The gene encoding the PH0300 TtuA protein from P. horikoshii was synthesized by GenScript with codon optimization for E. coli and subcloned into the pBG102 plasmid (pET27 derivative) between the BamHI and EcoRI restriction sites to produce a 6His-SUMO-TtuA protein construct.	Get A Quote