Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | A competitive enzyme linked immunosorbent assay (ELISA) was per-formed using a His-tag detection kit, following manufacturer'sinstructions (Genscript, Piscataway, NJ). | Get A Quote |
For therapies targeting diseases of the gastrointestinal tract, we and others envision probiotic bac-teria that synthesize and excrete biotherapeutics at disease sites. Toward this goal, we have engi-neered commensalE. colithat selectively synthesize and secrete a model biotherapeutic in thepresence of nitric oxide (NO), an intestinal biomarker for Crohn's disease (CD). This is accomplishedby co-expressing the pore forming protein TolAIII with the biologic, granulocyte macrophage-colony stimulating factor (GM-CSF). We have additionally engineered these bacteria to accumulateat sites of elevated NO by engineering their motility circuits and controlling pseudotaxis. Impor-tantly, because we have focused onin vit... More